Cocultivation of caprine arthritis encephalitis virus-infected macrophages with primary goat synovial cells
نویسندگان
چکیده
Supachart PANNEUM, Wilairat CHUMSING, Theera RUKKWAMSUK* Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand Center of Excellence on Agricultural Biotechnology (AG_BIO/PERDO-CHE), Bangkok, Thailand
منابع مشابه
Isolation and characterization of caprine arthritis encephalitis virus in goats from Poland.
The caprine arthritis-encephalitis virus (CAEV) was isolated from monocyte-derived macrophages (M/M), but not from PBMC of seropositive goats by co-cultivation with goat synovial membrane cells. Out of eight M/M co-cultures, CAEV was evidenced by the syncytia formation and presence of proviral DNA in two and four cultures, respectively. Two virus isolates from co-cultures showing cytopathic eff...
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We previously reported that infection of goats with caprine arthritis encephalitis virus (CAEV) tat- proviral DNA or virus results in persistent infection, since the animals seroconverted and direct virus isolation from cultures of blood-derived macrophages was positive. In this study we wanted to determine whether goats injected with CAEV tat- proviral DNA or virus were protected against chall...
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Primate lentivirus (HIV and SIV) vpr accessory genes encode 12- to 14-kDa proteins which induce cell cycle arrest at the G2 phase of infected cells, preventing them from going through mitosis. Members of the HIV-2/SIVmac/SIVsmm group also encode a second closely related accessory protein called Vpx. Vpx and HIV Vpr are critical for virus replication in nondividing cells due to their participati...
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Ovine lentiviruses and caprine arthritis-encephalitis virus (CAEV) are prototypic lentiviruses that replicate predominantly in macrophages of infected animals. In situ hybridization of pathologically affected tissues from diseased animals has shown that viral RNA exists in permissive macrophages as well as in non-macrophage cell types that do not support productive virus replication. These find...
متن کاملHumoral immune response to caprine arthritis-encephalitis virus in goat herds
Caprine arthritis-encephalitis (CAE) is an infectious contagious disease of goats caused by single-stranded RNA virus belonging to the family Retroviridae genus Lentivirus [1]. Caprine arthritis-encephalitis virus infects monocytes where, thanks to the enzyme – reverse transcriptase – it changes into DNA provirus and becomes integrated into the host genome [2]. Such latent infection is life-lon...
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تاریخ انتشار 2017